RIKEN Center for Life Science Technologies


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To let you know about our research, this area contains 4 types of information about CLST; “Article”, “Videos”, “Event” and “Study”.
At “Article”, you can read articles on interviews and lectures, and you can enjoy the videos about CLST at “Videos”. If you want to meet and talk directly with the researcher, “Visit” give you some information of such events. You can find more difficult contents to know about our research deeply at “Study”.
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Labs & Technologies

Molecular Network Control Factors Development Unit

We try to develop molecules that intervene in cellular pathways and thus modulate cell functions.


* Due to the reorganization starting as new centers in April 2018, this laboratory is now belong to the Center for Biosystems Dynamics Research. As for the latest information, please see the following URL below.
> The webpage of Laboratory for Advanced Biomolecular Engineering, Center for Biosystems Dynamics Research

Unit Leader
Shunsuke Tagami  Ph.D.

Room C516, Central Building, Yokohama Campus
1-7-22 Suehiro-cho,Tsurumi-ku, Yokohama 230-0045
TEL: 045-503-9205


Research Area

This unit aims to engineer functional bio-macromolecules applicable to medical and basic biological researches, by integrating technologies in synthetic and structural biology including 1) in vitro reconstruction of target protein complexes, 2) in vitro evolution of peptide/RNA, 3) protein X-ray crystallography, and 4) structure based protein/drug engineering. Delivery systems for the engineered molecules will be also developed in collaboration with the Molecular Network Contraol Imaging Unit.

Main Publications List


Simple peptides derived from the ribosomal core potentiate RNA polymerase ribozyme function.

Tagami S, Attwater J, Holliger P.
Nat Chem., 9(4), 325-332 (2017).

Structural basis for promoter specificity switching of RNA polymerase by a phage factor

Tagami S, Sekine S, Minakhin L, Esyunina D, Akasaka R, Shirouzu M, Kulbachinskiy A, Severinov K, Yokoyama S.
Genes Dev, 28(5), 521-531 (2014).

Chemical fidelity of an RNA polymerase ribozyme.

Attwater J, Tagami S, Kimoto M, Butler K, Kool E, T, Wengel J, Herdewijn P, Hirao I, Holliger P.
Chem Sci, 4, 2804-2814 (2013).

Structural basis of transcription by bacterial and eukaryotic RNA polymerases.

Sekine S, Tagami S, Yokoyama S.
Curr Opin Struct Biol, 22(1), 110-118 (2012).

A novel conformation of RNA polymerase sheds light on the mechanism of transcription.

Tagami S, Sekine SI, Yokoyama S.
Transcription, 2(4), 162-167 (2011).

Crystal structure of bacterial RNA polymerase bound with a transcription inhibitor protein.

Tagami S, Sekine S, Kumarevel T, Hino N, Murayama Y, Kamegamori S, Yamamoto M, Sakamoto K, Yokoyama S.
Nature, 468(7326), 978-982 (2010).

Crystallization and preliminary X-ray crystallographic analysis of Thermus thermophilus transcription elongation complex bound to Gfh1.

Tagami S, Sekine S, Kumarevel T, Yamamoto M, Yokoyama S.
Acta Crystallogr Sect F Struct Biol Cryst Commun., 66(Pt1), 63-68 (2010).

Member  *concurrent

CLST was reorganized into three centers according to the RIKEN 4th Medium-Term Plan from April 1, 2018. For the latest information of Molecular Network Control Factors Development Unit, please visit the following websites.

> The webpage of Laboratory for Advanced Biomolecular Engineering, Center for Biosystems Dynamics Research [http://www.bdr.riken.jp/en/research/labs/tagami-s/index.html]