RIKEN Center for Life Science Technologies


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For Public

To let you know about our research, this area contains 4 types of information about CLST; “Article”, “Videos”, “Event” and “Study”.
At “Article”, you can read articles on interviews and lectures, and you can enjoy the videos about CLST at “Videos”. If you want to meet and talk directly with the researcher, “Visit” give you some information of such events. You can find more difficult contents to know about our research deeply at “Study”.
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Technologies of CLST

Cell-free system that recapitulates miRNA-mediated mRNA regulation.



Regulation of translation and mRNA degradation are based on a complex interplay of canonical factors, diverse RNA-binding proteins, and non-coding regulatory RNAs (e.g. microRNAs).  We have established an in vitro experimental system to recapitulate mechanisms of miRNA-mediated post-transcriptional control by utilizing a cell-free translation technique.


In this system, by adding hairpin-like miRNA precursors or siRNA type double-stranded RNAs, a guide strand of miRNAs are incorporated into Argonaute proteins, and miRISC complexes are formed.  The miRISCs bind to the target mRNAs and induce deadenylation and translation inhibition that eventually leads repression of protein synthesis.  Our cell-free system is a powerful tool in elucidating miRNA mechanisms.


Related labs

CLST was reorganized into three centers according to the RIKEN 4th Medium-Term Plan from April 1, 2018.