RIKEN Center for Life Science Technologies


  • Research

    Latest publications from CLST.
  • News

    Awards, agreements, and other news.
  • Events

    Upcoming and past events including seminars, symposiums, open days.

For Public

To let you know about our research, this area contains 4 types of information about CLST; “Article”, “Videos”, “Event” and “Study”.
At “Article”, you can read articles on interviews and lectures, and you can enjoy the videos about CLST at “Videos”. If you want to meet and talk directly with the researcher, “Visit” give you some information of such events. You can find more difficult contents to know about our research deeply at “Study”.
  • Article
  • Event
  • Videos
  • Study

Labs & Technologies

Protein Functional and Structural Biology Team

Establishment of a structural analysis technology platform that contributes to "life innovation" such as drug development and medical treatment.


* Due to the reorganization starting as new centers in April 2018, this laboratory is now belong to the Center for Biosystems Dynamics Research. As for the latest information, please see the following URL below.
> The webpage of Laboratory for Protein Functional and Structural Biology, Center for Biosystems Dynamics Research

Team Leader
Mikako Shirouzu  Ph.D.

#W209 West Research Building, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama City, Kanagawa, 230-0045, Japan
Tel: +81-(0)45-503-9202


Research Area

We plan to establish a structural analysis technology platform to contribute to "life innovation" such as drug discovery and medical treatment as well as develop methods for the sample preparation and crystallization of challenging proteins including membrane proteins that have been difficult in its analysis. The high-resolution structural information of proteins related to diseases shall increasingly become important for drug development leading to future individualized medicine such as a molecular target drug. Especially, we will perform structural analysis mainly for proteins related to immune system diseases and kinases associated with cancer/leukemia. The 3D-structural information of these target proteins will be used for in silico screening of chemical compounds. Further analysis of the complex of the target protein with candidate compounds will provide valuable information for drug discovery.

Main Publications List


Na+-mimicking ligands stabilize the inactive state of leukotriene B4 receptor BLT1.

Hori T, Okuno T, Hirata K, Yamashita K, Kawano Y, Yamamoto M, Hato M, Nakamura M, Shimizu T, Yokomizo T, Miyano M, Yokoyama S.
Nat Chem Biol., 14(3), 262-269 (2018).

Cell-free synthesis of functional antibody fragments to provide a structural basis for antibody-antigen interaction.

Matsuda T, Ito T, Takemoto C, Katsura K, Ikeda M, Wakiyama M, Kukimoto-Niino M, Yokoyama S, Kurosawa Y, Shirouzu M,.
PLoS One, 13(2), e0193158 (2018).

Structural Insights into the Altering Function of CRMP2 by Phosphorylation.

Sumi T, Imasaki T, Aoki M, Sakai N, Nitta E, Shirouzu M, Nitta R.
Cell Struct Funct., 43(1), 15-23 (2018).

Identification of pyrrolo[2,3-d]pyrimidines as potent HCK and FLT3-ITD dual inhibitors.

Koda Y, Kikuzato K, Mikuni J, Tanaka A, Yuki H, Honma T, Tomabechi Y, Kukimoto-Niino M, Shirouzu M, Shirai F, Koyama H.
Bioorg Med Chem Lett., 27(22), 4944-4998 (2017).

Crystallization and X-ray analysis of 23 nm virus-like particles from Norovirus Chiba strain.

Hasegawa K, Someya Y, Shigematsu H, Kimura-Someya T, Nuemket N, Kumasaka T.
Acta Crystallogr F Struct Biol Commun., 73(Pt 10), 568-573 (2017).

Structural basis for CRMP2-induced axonal microtubule formation.

Niwa S, Nakamura F, Tomabechi Y, Aoki M, Shigematsu H, Matsumoto T, Yamagata A, Fukai S, Hirokawa N, Goshima Y, Shirouzu M, Nitta R
Sci Rep., 7(1), 10681 (2017).

Dynamic functional assembly of the Torsin AAA+ ATPase and its modulation by LAP1.

Chase AR, Laudermilch E, Wang J, Shigematsu H, Yokoyama T, Schlieker C.
Mol Biol Cell, 28(21), 2765-2772 (2017).

Backbone and side chain assignments of the second RNA-binding domain of Musashi-1 in its free form and in complex with 5-mer RNA.

Iwaoka R, Nagata T, Tsuda K, Imai T, Okano H, Kobayashi N, Katahira M.
Biomol NMR Assign., 11(2), 265-268 (2017).

Structure of the complete elongation complex of RNA polymerase II with basal factors.

Ehara H, Yokoyama T, Shigematsu H, Yokoyama S, Shirouzu M, Sekine SI.
Science., 357(6354), 921-924 (2017).

Structural Insight into the Recognition of r(UAG) by Musashi-1 RBD2, and Construction of a Model of Musashi-1 RBD1-2 Bound to the Minimum Target RNA.

Iwaoka R, Nagata T, Tsuda K, Imai T, Okano H, Kobayashi N, Katahira M.
Molecules., 22(7), E1207 (2017).

Parallel homodimer structures of the extracellular domains of the voltage-gated sodium channel β4 subunit explain its role in cell-cell adhesion.

Shimizu H, Tosaki A, Ohsawa N, Ishizuka-Katsura Y, Shoji S, Miyazaki H, Oyama F, Terada T, Shirouzu M, Sekine SI, Nukina N, Yokoyama S.
J Biol Chem., 292(32), 13428-13440 (2017).

Crystal structural characterization reveals novel oligomeric interactions of human voltage-dependent anion channel 1.

Hosaka T, Okazaki M, Kimura-Someya T, Ishizuka-Katsura Y, Ito K, Yokoyama S, Dodo K, Sodeoka M, Shirouzu M.
Protein Sci., 26(9), 1749-1758 (2017).

>> All Publications

Member  *concurrent

  • >>>Curriculum vitae
  • >>>Curriculum vitae
  • >>>Curriculum vitae

CLST was reorganized into three centers according to the RIKEN 4th Medium-Term Plan from April 1, 2018. For the latest information of Protein Functional and Structural Biology Team, please visit the following websites.

> The webpage of Laboratory for Protein Functional and Structural Biology, Center for Biosystems Dynamics Research [http://www.bdr.riken.jp/en/research/labs/shirouzu-m-protein/index.html]]